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Please use this identifier to cite or link to this item: http://arks.princeton.edu/ark:/88435/dsp01m039k7632
Title: Characterization of C. elegans Reproductive-Extension Genes repx-1 and far-2
Authors: Reed, Rachel
Advisors: Murphy, Coleen T
Department: Molecular Biology
Class Year: 2018
Abstract: Reproductive decline in females is one of the earliest signs of aging in the human population. With more women having children later in life, understanding the genetic and molecular causes of reproductive decline is becoming increasingly important. C. elegans, a commonly-studied nematode, also experience a similar reproductive decline marked by decreasing oocyte quality. A mutagenesis experiment and reproductive screen were completed to identify new components of the molecular pathways that control reproductive aging—two notable mutants were identified: repx-1 and ¬far-2. Further characterization of these two genes unveiled their effect on improving oocyte quality with age—repx-1 acts to extend reproductive span through decrease in oocyte quality decline without extending the overall lifespan of the C. elegans. Transcriptional profiles of these mutants were also generated using microarray analysis to create a preliminary list of significantly up-regulated and down-regulated genes.
URI: http://arks.princeton.edu/ark:/88435/dsp01m039k7632
Type of Material: Princeton University Senior Theses
Language: en
Appears in Collections:Molecular Biology, 1954-2023

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